Yeast Heterochromatin Only Stably Silences Weak Regulatory Elements by Altering Burst Duration.

ABSTRACT: The interplay between nucleosomes and transcription factors leads to programs of gene expression. Transcriptional silencing involves the generation of a chromatin state that represses transcription and is faithfully propagated through DNA replication and cell division. Using multiple reporter assays, including directly visualizing transcription in single cells, we investigated a diverse set of UAS enhancers and core promoters for their susceptibility to heterochromatic gene silencing. These results show that heterochromatin only stably silences weak and stress induced regulatory elements but is unable to stably repress housekeeping gene regulatory elements and the partial repression did not result in bistable expression states. Permutation analysis of different UAS enhancers and core promo

SUBMITTER: Wu K 

PROVIDER: S-EPMC10592971 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature