Compact Programmable Control of Protein Secretion in Mammalian Cells.

ABSTRACT: Synthetic biology currently holds immense potential to engineer the spatiotemporal control of intercellular signals for biomedicine. Programming behaviors using protein-based circuits has advantages over traditional gene circuits such as compact delivery and direct interactions with signaling proteins. Previously, we described a generalizable platform called RELEASE to enable the control of intercellular signaling through the proteolytic removal of ER-retention motifs compatible with pre-existing protease-based circuits. However, these tools lacked the ability to reliably program complex expression profiles and required numerous proteases, limiting delivery options. Here, we harness the recruitment and antagonistic behavior of endogenous 14-3-3 proteins to create RELEASE-NOT to turn off pr

SUBMITTER: Vlahos AE 

PROVIDER: S-EPMC10592972 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature