De Novo Biosynthesis of Vindoline and Catharanthine in Saccharomyces cerevisiae.

ABSTRACT: Vinblastine has been used clinically as one of the most potent therapeutics for the treatment of several types of cancer. However, the traditional plant extraction method suffers from unreliable supply, low abundance, and extremely high cost. Here, we use synthetic biology approach to engineer Saccharomyces cerevisiae for de novo biosynthesis of vindoline and catharanthine, which can be coupled chemically or biologically to vinblastine. On the basis of a platform strain with sufficient supply of precursors and cofactors for biosynthesis, we reconstituted, debottlenecked, and optimized the biosynthetic pathways for the production of vindoline and catharanthine. The vindoline biosynthetic pathway represents one of the most complicated pathways ever reconstituted in microbial cell fact

SUBMITTER: Gao D 

PROVIDER: S-EPMC10593122 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature