Side-chain dynamics of the α_1B -adrenergic receptor determined by NMR via methyl relaxation.

ABSTRACT: G protein-coupled receptors (GPCRs) are medically important membrane proteins that sample inactive, intermediate, and active conformational states characterized by relatively slow interconversions (~μs-ms). On a faster timescale (~ps-ns), the conformational landscape of GPCRs is governed by the rapid dynamics of amino acid side chains. Such dynamics are essential for protein functions such as ligand recognition and allostery. Unfortunately, technical challenges have almost entirely precluded the study of side-chain dynamics for GPCRs. Here, we investigate the rapid side-chain dynamics of a thermostabilized α_1B -adrenergic receptor (α_1B -AR) as probed by methyl relaxation. We determined order parameters for Ile, Leu, and Val methyl groups in the presence of inverse ago

SUBMITTER: Baumann C 

PROVIDER: S-EPMC10593183 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature