Optimizing insect metabarcoding using replicated mock communities.

ABSTRACT: 1: Metabarcoding (high-throughput sequencing of marker gene amplicons) has emerged as a promising and cost-effective method for characterizing insect community samples. Yet, the methodology varies greatly among studies and its performance has not been systematically evaluated to date. In particular, it is unclear how accurately metabarcoding can resolve species communities in terms of presence-absence, abundances, and biomass. 2: Here we use mock community experiments and a simple probabilistic model to evaluate the effect of different DNA extraction protocols on metabarcoding performance. Specifically, we ask four questions: (Q1) How consistent are the recovered community profiles across replicate mock communities?; (Q2) How does the choice of lysis buffer affect the recovery of the origi

SUBMITTER: Iwaszkiewicz-Eggebrecht E 

PROVIDER: S-EPMC10593422 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature