Comparative Proteomic Analysis of Two Commonly Used Laboratory Yeast Strains: W303 and BY4742.

ABSTRACT: The yeast Saccharomyces cerevisiae is a powerful model system that is often used to expand our understanding of cellular processes and biological functions. Although many genetically well-characterized laboratory strains of S. cerevisiae are available, they may have different genetic backgrounds which can confound data interpretation. Here, we report a comparative whole-proteome analysis of two common laboratory yeast background strains, W303 and BY4742, in both exponential and stationary growth phases using isobaric-tag-based mass spectrometry to highlight differences in proteome complexity. We quantified over 4400 proteins, hundreds of which showed differences in abundance between strains and/or growth phases. Moreover, we used proteome-wide protein abundance to profile the

SUBMITTER: Rossio V 

PROVIDER: S-EPMC10594481 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature