Comparison of the Proteomes and Phosphoproteomes of S. cerevisiae Cells Harvested with Different Strategies.

ABSTRACT: The budding yeast Saccharomyces cerevisiae is a powerful model system that is widely used to investigate many cellular processes. The harvesting of yeast cells is the first step in almost every experimental procedure. Here, yeast cells are isolated from their growth medium, collected, and used for successive experiments or analysis. The two most common methods to harvest S. cerevisiae are centrifugation and filtration. Understanding if and how centrifugation and filtration affect yeast physiology is essential with respect to downstream data interpretation. Here, we profile and compare the proteomes and the phosphoproteomes, using isobaric label-based quantitative mass spectrometry, of three common methods used to harvest S. cerevisiae cells: low-speed centrifugation, h

SUBMITTER: Rossio V 

PROVIDER: S-EPMC10594529 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature