Assessment of Four Engineered PET Degrading Enzymes Considering Large-Scale Industrial Applications.

ABSTRACT: In recent years, enzymatic recycling of the widely used polyester polyethylene terephthalate (PET) has become a complementary solution to current thermomechanical recycling for colored, opaque, and mixed PET. A large set of promising hydrolases that depolymerize PET have been found and enhanced by worldwide initiatives using various methods of protein engineering. Despite the achievements made in these works, it remains difficult to compare enzymes' performance and their applicability to large-scale reactions due to a lack of homogeneity between the experimental protocols used. Here, we pave the way for a standardized enzymatic PET hydrolysis protocol using reaction conditions relevant for larger scale hydrolysis and apply these parameters to four recently reported PET hydrolases (LCC

SUBMITTER: Arnal G 

PROVIDER: S-EPMC10594578 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature