A Minispidroin Guides the Molecular Design for Cellular Condensation Mechanisms in S. cerevisiae.

ABSTRACT: Structural engineering of molecules for condensation is an emerging technique within synthetic biology. Liquid-liquid phase separation of biomolecules leading to condensation is a central step in the assembly of biological materials into their functional forms. Intracellular condensates can also function within cells in a regulatory manner to facilitate reaction pathways and to compartmentalize interactions. We need to develop a strong understanding of how to design molecules for condensates and how their in vivo-in vitro properties are related. The spider silk protein NT2RepCT undergoes condensation during its fiber-forming process. Using parallel in vivo and in vitro characterization, in this study, we mapped the effects of intracellular conditions for NT2RepC

SUBMITTER: Feng J 

PROVIDER: S-EPMC10594646 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature