Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks.

ABSTRACT:

Background

Proteins related to sperm motility and sperm morphology have an important impact on sperm function such as metabolism, motility and fertilisation etc. An understanding of the key proteins related to semen quality in Niangya yaks would help to provide support for breeding. However, the key proteins that affect semen quality in Niangya yaks remain unclear.

Methods

Herein, we applied tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC‒MS/MS) to analyze the expression levels of sperm proteins in groups of high- and low-quality semen from Niangya yaks. And fifteen differentially expressed proteins (DEPs) were randomly selected for expression level validation by parallel reaction monitoring (PRM).

Results

Of the 2,092 quantified

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10594827 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature