Untangling an insect's virome from its endogenous viral elements.

ABSTRACT:

Background

Insects are an important reservoir of viral biodiversity, but the vast majority of viruses associated with insects have not been discovered. Recent studies have employed high-throughput RNA sequencing, which has led to rapid advances in our understanding of insect viral diversity. However, insect genomes frequently contain transcribed endogenous viral elements (EVEs) with significant homology to exogenous viruses, complicating the use of RNAseq for viral discovery.

Methods

In this study, we used a multi-pronged sequencing approach to study the virome of an important agricultural pest and prolific vector of plant pathogens, the potato aphid Macrosiphum euphorbiae. We first used rRNA-depleted RNAseq to characterize the microbes found in individual insects. We then u

SUBMITTER: Rozo-Lopez P 

PROVIDER: S-EPMC10594914 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature