K⁺/Cl^- cotransporter 2 (KCC2) and Na⁺/HCO3- cotransporter 1 (NBCe1) interaction modulates profile of KCC2 phosphorylation.

ABSTRACT: K⁺/Cl^- cotransporter 2 (KCC2) is a major Cl^- extruder in mature neurons and is responsible for the establishment of low intracellular [Cl^-], necessary for fast hyperpolarizing GABA_A-receptor mediated synaptic inhibition. Electrogenic sodium bicarbonate cotransporter 1 (NBCe1) is a pH regulatory protein expressed in neurons and glial cells. An interactome study identified NBCe1 as a possible interaction partner of KCC2. In this study, we investigated the putative effect of KCC2/NBCe1 interaction in baseline and the stimulus-induced phosphorylation pattern and function of KCC2. Primary mouse hippocampal neuronal cultures from wildtype (WT) and Nbce1-deficient mice, as well as HEK-293 cells stably transfected with KCC2^WT

SUBMITTER: Pethe A 

PROVIDER: S-EPMC10595033 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature