Development of Cas13a-based assays for Neisseria gonorrhoeae detection and gyrase A determination.

ABSTRACT: Neisseria gonorrhoeae is one of the most common bacterial sexually transmitted infections. The emergence of antimicrobial-resistant N. gonorrhoeae is an urgent public health threat. Currently, the diagnosis of N. gonorrhoeae infection requires expensive laboratory infrastructure, while antimicrobial susceptibility determination requires bacterial culture, both of which are infeasible in low-resource areas where the prevalence of infection is highest. Recent advances in molecular diagnostics, such as specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) using CRISPR-Cas13a and isothermal amplification, have the potential to provide low-cost detection of pathogen and antimicrobial resistance. We designed and optimized RNA gu

SUBMITTER: Allan-Blitz L-T 

PROVIDER: S-EPMC10597441 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature