Differential innate immune responses of human macrophages and bronchial epithelial cells against Talaromyces marneffei.

ABSTRACT: Talaromyces marneffei is a thermally dimorphic fungal pathogen endemic in Southeast Asia. As inhalation of airborne conidia is believed as the major infection route, airway epithelial cells followed by pulmonary macrophages are the first cell types which the fungus encounters inside the host. In this study, we established an in vitro infection model based on human peripheral blood-derived macrophages (hPBDMs) cultured with the supplementation of autologous plasma. Using this model, we determined the transcriptomic changes of hPBDMs in response to T. marneffei infection by quantitative real-time reverse-transcription polymerase chain reaction as well as high-throughput RNA sequencing. Results showed that T. marneffei infection could activate hPBDMs to the M1-like

SUBMITTER: Tan Y-P 

PROVIDER: S-EPMC10597461 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature