Host-pathogen interaction: Enterobacter cloacae exerts different adhesion and invasion capacities against different host cell types.

ABSTRACT: New antibiotics are urgently needed due to the huge increase of multidrug-resistant bacteria. The underexplored gram-negative bacterium Enterobacter cloacae is known to cause severe urinary tract and lung infections (UTIs). The pathogenicity of E. cloacae in UTI has only been studied at the bioinformatic level, but until now not within systematic in vitro investigations. The present study assesses different human cell lines for monitoring the early steps of host-pathogen interaction regarding bacterial adhesion to and invasion into different host cells by flow cytometric adhesion assay, classical cell counting assay, gentamicin invasion assay, and confocal laser scanning microscopy. To our knowledge, this is the first report in which E. cloacae has been investigated for its interaction wit

SUBMITTER: Frutos-Grilo E 

PROVIDER: S-EPMC10597508 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature