An ex vivo rat trachea model reveals abnormal airway physiology and a gland secretion defect in cystic fibrosis.

ABSTRACT: Cystic fibrosis (CF) is a genetic disease hallmarked by aberrant ion transport that results in delayed mucus clearance, chronic infection, and progressive lung function decline. Several animal models have been developed to study the airway anatomy and mucus physiology in CF, but they are costly and difficult to maintain, making them less accessible for many applications. A more available CFTR-/- rat model has been developed and characterized to develop CF airway abnormalities, but consistent dosing of pharmacologic agents and longitudinal evaluation remain a challenge. In this study, we report the development and characterization of a novel ex vivo trachea model that utilizes both wild type (WT) and CFTR-/- rat tracheae cultured on a porcine gelatin matrix. Here we show that the ex vivo tr

SUBMITTER: Harris E 

PROVIDER: S-EPMC10597513 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature