Fabrication of membrane proteins in the form of native cell membrane nanoparticles using novel membrane active polymers.

ABSTRACT: Membrane proteins are a widespread class of bio-macromolecules responsible for numerous vital biological processes and serve as therapeutic targets for a vast array of contemporary medications. For membrane protein isolation and purification, detergents have historically been used. Despite this, detergents frequently result in protein instability. Consequently, their application was limited. Recent detergent-free approaches have been invented. Among these, styrene-maleic acid lipid particle (SMALP), diisobutylene-maleic acid lipid particle (DIBMALP), and native cell membrane nanoparticle (NCMN) systems are the most prevalent. The NCMN system intends to create a library of membrane-active polymers suitable for high-resolution structure determination of membrane protein. Design, synthesis, c

SUBMITTER: Hoang Trinh TK 

PROVIDER: S-EPMC10597567 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature