Project description:Soft x-ray tomography (SXT) is increasingly being recognized as a valuable method for visualizing and quantifying the ultrastructure of cryopreserved cells. Here, we describe the combination of SXT with cryogenic confocal fluorescence tomography (CFT). This correlative approach allows the incorporation of molecular localization data, with isotropic precision, into high-resolution three-dimensional (3-D) SXT reconstructions of the cell. CFT data are acquired first using a cryogenically adapted confocal light microscope in which the specimen is coupled to a high numerical aperture objective lens by an immersion fluid. The specimen is then cryo-transferred to a soft x-ray microscope (SXM) for SXT data acquisition. Fiducial markers visible in both types of data act as common landmarks, enabling accurate coalignment of the two complementary tomographic reconstructions. We used this method to identify the inactive X chromosome (Xi) in female v-abl transformed thymic lymphoma cells by localizing enhanced green fluorescent protein-labeled macroH2A with CFT. The molecular localization data were used to guide segmentation of Xi in the SXT reconstructions, allowing characterization of the Xi topological arrangement in near-native state cells. Xi was seen to adopt a number of different topologies with no particular arrangement being dominant.

| S-EPMC4213715 | biostudies-literature

Ultra-flat and long-lived plasmons in a strongly correlated oxide.

Project description:Plasmons in strongly correlated systems are attracting considerable attention due to their unconventional behavior caused by electronic correlation effects. Recently, flat plasmons with nearly dispersionless frequency-wave vector relations have drawn significant interest because of their intriguing physical origin and promising applications. However, these flat plasmons exist primarily in low-dimensional materials with limited wave vector magnitudes (q < ~0.7 Å-1). Here, we show that long-lived flat plasmons can propagate up to ~1.2 Å-1 in α-Ti2O3, a strongly correlated three-dimensional Mott-insulator, with an ultra-small energy fluctuation (<40 meV). The strong correlation effect renormalizes the electronic bands near Fermi level with a small bandwidth, which is responsible for the flat plasmons in α-Ti2O3. Moreover, these flat plasmons are not affected by Landau damping over a wide range of wave vectors (q < ~1.2 Å-1) due to symmetry constrains on the electron wavefunctions. Our work provides a strategy for exploring flat plasmons in strongly correlated systems, which in turn may give rise to novel plasmonic devices in which flat and long-lived plasmons are desirable.

| S-EPMC9363501 | biostudies-literature

Tunable and low-loss correlated plasmons in Mott-like insulating oxides.

Project description:Plasmonics has attracted tremendous interests for its ability to confine light into subwavelength dimensions, creating novel devices with unprecedented functionalities. New plasmonic materials are actively being searched, especially those with tunable plasmons and low loss in the visible-ultraviolet range. Such plasmons commonly occur in metals, but many metals have high plasmonic loss in the optical range, a main issue in current plasmonic research. Here, we discover an anomalous form of tunable correlated plasmons in a Mott-like insulating oxide from the Sr1-xNb1-yO3+δ family. These correlated plasmons have multiple plasmon frequencies and low loss in the visible-ultraviolet range. Supported by theoretical calculations, these plasmons arise from the nanometre-spaced confinement of extra oxygen planes that enhances the unscreened Coulomb interactions among charges. The correlated plasmons are tunable: they diminish as extra oxygen plane density or film thickness decreases. Our results open a path for plasmonics research in previously untapped insulating and strongly-correlated materials.

| S-EPMC5437299 | biostudies-literature

Soft plasmons with stretchable spectroscopic response based on thermally patterned gold nanoparticles.

Project description:Flexible photonic crystals are attractive devices owing to their multifold tunable parameters additionally introduced by soft substrates or by nanostructured, nano-doped, or nano-embedded soft matters. This not only extends significantly the intrinsic functions of photonic crystals, but also facilitates easy integration of the photonic crystal device into various optoelectronic and sensing systems. So far, flexible metallic photonic structures have been constructed on micrometer scales with complex fabrication procedures. Much simpler and more reproducible methods are expected to achieve such metamaterials in large scales and at low costs. In address to these challenges, we developed a straightforward approach to create soft plasmonic photonic crystals consisting of gold nanolines arranged on stretchable substrates with nanoscale periods, centimeter-scale areas, and high reproducibility using annealed gold nanoparticle colloids.

| S-EPMC3933943 | biostudies-literature

Comparison between trabectedin and doxorubicin in soft-tissue sarcomas-reply letter.

Project description: Not available

| S-EPMC9073790 | biostudies-literature

Reply to: On the existence of collective interactions reinforcing the metal-ligand bond in organometallic compounds.

Project description: Not available

| S-EPMC10317954 | biostudies-literature

Reconstructed cryo soft X-ray tomography dataset of a treated NIH-3T3 cell with corresponding correlated cryo-3D-SIM channels

Project description: Not available

| EMPIAR-10839 | biostudies-other

0.5-keV Soft X-ray attosecond continua.

Project description:Attosecond light pulses in the extreme ultraviolet have drawn a great deal of attention due to their ability to interrogate electronic dynamics in real time. Nevertheless, to follow charge dynamics and excitations in materials, element selectivity is a prerequisite, which demands such pulses in the soft X-ray region, above 200 eV, to simultaneously cover several fundamental absorption edges of the constituents of the materials. Here, we experimentally demonstrate the exploitation of a transient phase matching regime to generate carrier envelope controlled soft X-ray supercontinua with pulse energies up to 2.9±0.1 pJ and a flux of (7.3±0.1) × 10(7) photons per second across the entire water window and attosecond pulses with 13 as transform limit. Our results herald attosecond science at the fundamental absorption edges of matter by bridging the gap between ultrafast temporal resolution and element specific probing.

| S-EPMC4865833 | biostudies-literature

PSF correction in soft X-ray tomography.

Project description:In this article, we introduce a linear approximation of the forward model of soft X-ray tomography, such that the reconstruction is solvable by standard iterative schemes. This linear model takes into account the three-dimensional point spread function (PSF) of the optical system, which consequently enhances the reconstruction of data. The feasibility of the model is demonstrated on both simulated and experimental data, based on theoretically estimated and experimentally measured PSFs.

| S-EPMC6371978 | biostudies-literature

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