Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi.

ABSTRACT: Obtaining Plasmodium vivax sporozoites is essential for in vitro culture of liver stage parasites, not only to understand fundamental aspects of parasite biology, but also for drug and vaccine development. A major impediment to establish high-throughput in vitro P. vivax liver stage assays for drug development is obtaining sufficient numbers of sporozoites. To do so, female anopheline mosquitoes have to be fed on blood from P. vivax-infected patients through an artificial membrane-feeding system, which in turns requires a well-established Anopheles colony. In this study we established conditions to provide a robust supply of P. vivax sporozoites. Adding a combination of serum replacement and antibiotics to the membrane-feeding protocol was found to best improve sporozoite production. A sim

SUBMITTER: Andrade AO 

PROVIDER: S-EPMC10598059 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature