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Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality.

ABSTRACT: Process development for transferring lab-scale research workflows to automated manufacturing procedures is critical for chimeric antigen receptor (CAR)-T cell therapies. Therefore, the key factor for cell viability, expansion, modification, and functionality is the optimal combination of medium and T cell activator as well as their regulatory compliance for later manufacturing under Good Manufacturing Practice (GMP). In this study, we compared two protocols for CAR-mRNA-modified T cell generation using our current lab-scale process, analyzed all mentioned parameters, and evaluated the protocols' potential for upscaling and process development of mRNA-based CAR-T cell therapies.

SUBMITTER: von Auw N

PROVIDER: S-EPMC10598065 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality.

von Auw Nadine N Serfling Robert R Kitte Reni R Hilger Nadja N Zhang Chengkang C Gebhardt Clara C Duenkel Anna A Franz Paul P Koehl Ulrike U Fricke Stephan S Tretbar U Sandy US

Scientific reports 20231024 1

Process development for transferring lab-scale research workflows to automated manufacturing procedures is critical for chimeric antigen receptor (CAR)-T cell therapies. Therefore, the key factor for cell viability, expansion, modification, and functionality is the optimal combination of medium and T cell activator as well as their regulatory compliance for later manufacturing under Good Manufacturing Practice (GMP). In this study, we compared two protocols for CAR-mRNA-modified T cell generatio ...[more]

PMID: 37875523

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