Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase.

ABSTRACT: Biomolecular polyelectrolyte complexes can be formed between oppositely charged intrinsically disordered regions (IDRs) of proteins or between IDRs and nucleic acids. Highly charged IDRs are abundant in the nucleus, yet few have been functionally characterized. Here, we show that a positively charged IDR within the human ATP-dependent DNA helicase Q4 (RECQ4) forms coacervates with G-quadruplexes (G4s). We describe a three-step model of charge-driven coacervation by integrating equilibrium and kinetic binding data in a global numerical model. The oppositely charged IDR and G4 molecules form a complex in the solution that follows a rapid nucleation-growth mechanism leading to a dynamic equilibrium between dilute and condensed phases. We also discover a physical interaction with Replication P

SUBMITTER: Papageorgiou AC 

PROVIDER: S-EPMC10598209 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature