Islets-on-Chip: A Tool for Real-Time Assessment of Islet Function Prior to Transplantation.

ABSTRACT: Islet transplantation improves metabolic control in patients with unstable type 1 diabetes. Clinical outcomes have been improving over the last decade, and the widely used beta-score allows the evaluation of transplantation results. However, predictive pre-transplantation criteria of islet quality for clinical outcomes are lacking. In this proof-of-concept study, we examined whether characterization of the electrical activity of donor islets could provide a criterion. Aliquots of 8 human donor islets from the STABILOT study, sampled from islet preparations before transplantation, were characterized for purity and split for glucose-induced insulin secretion and electrical activity using multi-electrode-arrays. The latter tests glucose concentration dependencies, biphasic activity, hormones,

SUBMITTER: Raoux M 

PROVIDER: S-EPMC10598278 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature