Fluorophore position of headgroup-labeled Gb₃ glycosphingolipids in lipid bilayers.

ABSTRACT: Fluorescent lipid probes are an invaluable tool for investigating lipid membranes. In particular, localizing certain receptor lipids such as glycosphingolipids within phase-separated membranes is of pivotal interest to understanding the influence of protein-receptor lipid binding on membrane organization. However, fluorescent labeling can readily alter the phase behavior of a lipid membrane because of the interaction of the fluorescent moiety with the membrane interface. Here, we investigated Gb₃ glycosphingolipids, serving as receptor lipids for the protein Shiga toxin, with a headgroup attached BODIPY fluorophore separated by a polyethylene glycol (PEG) spacer of different lengths. We found that the diffusion coefficients of the fluorescently labeled Gb₃ species in

SUBMITTER: Socrier L 

PROVIDER: S-EPMC10598288 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature