Autocrine regulation of tumor cell repopulation by Hsp70-HMGB1 alarmin complex.

ABSTRACT:

Background

Cancer recurrence is regulated by a variety of factors, among which is the material of dying tumor cells; it is suggested that remaining after anti-cancer therapy tumor cells receive a signal from proteins called damage-associated molecular patterns (DAMPs), one of which is heat shock protein 70 (Hsp70).

Methods

Two models of tumor repopulation were employed, based on minimal population of cancer cells and application of conditioned medium (CM). To deplete the CMs of Hsp70 affinity chromatography on ATP-agarose and immunoprecipitation were used. Cell proliferation and the dynamics of cell growth were measured using MTT assay and xCELLigence technology; cell growth markers were estimated using qPCR and with the aid of ELISA for prostaglandin E detection. Immunoprec

SUBMITTER: Sverchinsky DV 

PROVIDER: S-EPMC10598926 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature