Brequinar inhibits African swine fever virus replication in vitro by activating ferroptosis.

ABSTRACT:

Background

African swine fever virus (ASFV) is one of the most fatal swine etiological agents and has a huge economic impact on the global pork industry. Given that no effective vaccines or anti-ASFV drugs are available, there remains a pressing need for novel anti-ASFV drugs. This study aimed to investigate the anti-African swine fever virus (ASFV) activity of brequinar, a DHODH inhibitor.

Methods

The anti-ASFV activity of brequinar was investigated using IFA, HAD, HAD₅₀, qRT-PCR, and western blotting assays. The western blotting assay was used to investigate whether brequinar inhibits ASFV replication by killing ASFV particles directly or by acting on cell factors. The confocal microscopy and western blotting assays were used to investigate whether brequinar inh

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10599058 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature