Molecular basis of sulfolactate synthesis by sulfolactaldehyde dehydrogenase from <i>Rhizobium leguminosarum</i>.

ABSTRACT: Sulfolactate (SL) is a short-chain organosulfonate that is an important reservoir of sulfur in the biosphere. SL is produced by oxidation of sulfolactaldehyde (SLA), which in turn derives from sulfoglycolysis of the sulfosugar sulfoquinovose, or through oxidation of 2,3-dihydroxypropanesulfonate. Oxidation of SLA is catalyzed by SLA dehydrogenases belonging to the aldehyde dehydrogenase superfamily. We report that SLA dehydrogenase RlGabD from the sulfoglycolytic bacterium Rhizobium leguminsarum SRDI565 can use both NAD⁺ and NADP⁺ as cofactor to oxidize SLA, and indicatively operates through a rapid equilibrium ordered mechanism. We report the cryo-EM structure of RlGabD bound to NADH, revealing a tetrameric quaternary structure and supporting pro

SUBMITTER: Li J 

PROVIDER: S-EPMC10599462 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature