Nonribosomal peptides protect Pseudomonas nunensis 4A2e from amoebal and nematodal predation.

ABSTRACT: The rhizosphere is a highly competitive environment forcing bacteria to evolve strategies to oppose their enemies. The production of toxic secondary metabolites allows bacteria to counteract predators. In this study, we describe the anti-predator armamentarium of the soil-derived bacterium Pseudomonas nunensis 4A2e. Based on a genome mining approach, we identified several biosynthetic gene clusters coding for nonribosomal peptide synthetases. Generation of gene deletion mutants of the respective clusters shows a loss of defense capabilities. We isolated the novel lipopeptides keanumycin D and nunapeptins B and C, and fully elucidated their structures by a combination of in-depth mass spectrometry experiments, stable isotope labelling, and chemical synthesis. Additionally, investigat

SUBMITTER: Pflanze S 

PROVIDER: S-EPMC10599466 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature