Live while the DNA lasts. The role of autophagy in DNA loss and survival of diploid yeast cells during chronological aging.

ABSTRACT: Aging is inevitable and affects all cell types, thus yeast cells are often used as a model in aging studies. There are two approaches to studying aging in yeast: replicative aging, which describes the proliferative potential of cells, and chronological aging, which is used for studying post-mitotic cells. While analyzing the chronological lifespan (CLS) of diploid Saccharomyces cerevisiae cells, we discovered a remarkable phenomenon: ploidy reduction during aging progression. To uncover the mechanism behind this unusual process we used yeast strains undergoing a CLS assay, looking for various aging parameters. Cell mortality, regrowth ability, autophagy induction and cellular DNA content measurements indicated that during the CLS assay, dying cells lost their DNA, and only diploids

SUBMITTER: Enkhbaatar T 

PROVIDER: S-EPMC10599738 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature