Antiviral type III CRISPR signalling via conjugation of ATP and SAM.

ABSTRACT: CRISPR systems are widespread in the prokaryotic world, providing adaptive immunity against mobile genetic elements^1,2. Type III CRISPR systems, with the signature gene cas10, use CRISPR RNA to detect non-self RNA, activating the enzymatic Cas10 subunit to defend the cell against mobile genetic elements either directly, via the integral histidine-aspartate (HD) nuclease domain^3-5 or indirectly, via synthesis of cyclic oligoadenylate second messengers to activate diverse ancillary effectors^6-9. A subset of type III CRISPR systems encode an uncharacterized CorA-family membrane protein and an associated NrN family phosphodiesterase that are predicted to function in antiviral defence. Here we demonstrate that the CorA-associated type III-B (Cmr) CRISPR system f

SUBMITTER: Chi H 

PROVIDER: S-EPMC10600005 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature