Comparative genomics hints at dispensability of multiple essential genes in two Escherichia coli L-form strains.

ABSTRACT: Despite the critical role of bacterial cell walls in maintaining cell shapes, certain environmental stressors can induce the transition of many bacterial species into a wall-deficient state called L-form. Long-term induced Escherichia coli L-forms lose their rod shape and usually hold significant mutations that affect cell division and growth. Besides this, the genetic background of L-form bacteria is still poorly understood. In the present study, the genomes of two stable L-form strains of E. coli (NC-7 and LWF+) were sequenced and their gene mutation status was determined and compared with their parental strains. Comparative genomic analysis between two L-forms reveals both unique adaptions and common mutated genes, many of which belong to essential gene categories not involved in cell w

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10600066 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature