Single-cell morphological and topological atlas reveals the ecosystem diversity of human breast cancer.

ABSTRACT: Digital pathology allows computerized analysis of tumor ecosystem using whole slide images (WSIs). Here, we present single-cell morphological and topological profiling (sc-MTOP) to characterize tumor ecosystem by extracting the features of nuclear morphology and intercellular spatial relationship for individual cells. We construct a single-cell atlas comprising 410 million cells from 637 breast cancer WSIs and dissect the phenotypic diversity within tumor, inflammatory and stroma cells respectively. Spatially-resolved analysis identifies recurrent micro-ecological modules representing locoregional multicellular structures and reveals four breast cancer ecotypes correlating with distinct molecular features and patient prognosis. Further analysis with multiomics data uncovers clinically rele

SUBMITTER: Zhao S 

PROVIDER: S-EPMC10600153 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature