Antimicrobial peptidase lysostaphin at subinhibitory concentrations modulates staphylococcal adherence, biofilm formation, and toxin production.

ABSTRACT:

Background

The ability of antimicrobial agents to affect microbial adherence to eukaryotic cell surfaces is a promising antivirulence strategy for combating the global threat of antimicrobial resistance. Inadequate use of antimicrobials has led to widespread instances of suboptimal antibiotic concentrations around infection sites. Therefore, we aimed to examine the varying effect of an antimicrobial peptidase lysostaphin (APLss) on staphylococcal adherence to host cells, biofilm biomass formation, and toxin production as a probable method for mitigating staphylococcal virulence.

Results

Initially, soluble expression in E. coli and subsequent purification by immobilized-Ni²⁺ affinity chromatography (IMAC) enabled us to successfully produce a large quantity of highl

SUBMITTER: Yue Y 

PROVIDER: S-EPMC10601153 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature