EFR3A: a new raft domain organizing protein?

ABSTRACT:

Background

Membrane rafts play a crucial role in the regulation of many important biological processes. Our previous data suggest that specific interactions of flotillins with MPP1 are responsible for membrane raft domain organization and regulation in erythroid cells. Interaction of the flotillin-based protein network with specific membrane components underlies the mechanism of raft domain formation and regulation, including in cells with low expression of MPP1.

Methods

We sought to identify other flotillin partners via the immobilized recombinant flotillin-2-based affinity approach and mass spectrometry technique. The results were further confirmed via immunoblotting and via co-immunoprecipitation. In order to study the effect of the candidate protein on the physicochemica

SUBMITTER: Trybus M 

PROVIDER: S-EPMC10601247 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature