A universal CRISPR-Cas14a responsive triple-sensitized upconversion photoelectrochemical sensor.

ABSTRACT: It has recently been discovered that, like other members of the Cas family (12a and 13a), the clustered regularly interspaced short palindrome repeat CRISPR-Cas14a system not only mediates high-sensitivity detection with exceptionally strong gene editing ability but is also generally useful for DNA detection via fluorescence. Photoelectrochemical (PEC) sensors have been widely applied as efficient analytical tools. Measuring electrical signals is more cost-effective and the necessary equipment is more easily portable than fluorescence signal detectors, but their stability still needs to be improved. The high base resolution of CRISPR-Cas14a can compensate for such shortcomings. Therefore, electrical signals and fluorescence signals were combined, and the development of a universal CRISPR-C

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10601294 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature