Regulation of self-renewal and senescence in primitive mesenchymal stem cells by Wnt and TGFβ signaling.

ABSTRACT:

Background

The therapeutic application of multipotent mesenchymal stem cells (MSCs) encounters significant challenges, primarily stemming from their inadequate growth and limited self-renewal capabilities. Additionally, as MSCs are propagated, their ability to self-renew declines, and the exact cellular and molecular changes responsible for this are poorly understood. This study aims to uncover the complex molecular mechanisms that govern the self-renewal of primitive (p) MSCs.

Methods

We grew pMSCs using two types of medium, fetal bovine serum (FM) and xeno-free (XM), at both low passage (LP, P3) and high passage (HP, P20). To evaluate LP and HP pMSCs, we examined their physical characteristics, cell surface markers, growth rate, colony-forming ability, BrdU assays for prol

SUBMITTER: Mazzella M 

PROVIDER: S-EPMC10601332 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature