Establishment of novel immortalized middle ear cell lines as models for otitis media.

ABSTRACT:

Objective

Otitis media (OM) is among the most frequently diagnosed pediatric diseases in the US. Despite the significant public health burden of OM and the contribution research in culture models has made to understanding its pathobiology, a singular immortalized human middle ear epithelial (MEE) cell line exists (HMEEC-1, adult-derived). We previously developed MEE cultures from pediatric patients with non-inflamed MEE (PCI), recurrent OM (ROM), or OM with effusion (OME) and demonstrated differences in their baseline inflammatory cytokine expression and response to stimulation with an OM-relevant pathogen lysate and cytokines. Herein, we sought to immortalize these cultures and assess retention of their phenotypes.

Methods

MEE cultures were immortalized via lentivirus encod

SUBMITTER: Blaine-Sauer S 

PROVIDER: S-EPMC10601576 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature