Flow Cytometric Characterization of Hematopoietic Stem and Progenitor Cell Subpopulations in Autologous Peripheral Blood Stem Cell Preparations after Cryopreservation.

ABSTRACT:

Introduction

Autologous stem cell transplantation is a successful routine procedure with only a small number of non-engraftment cases, although the time to hematopoietic recovery may vary considerably across patients. While CD34 has been the decisive marker for enumerating hematopoietic stem and progenitor cells (HSPCs) for more than 30 years, the impact of CD34-positive cellular subpopulations in autologous HSPC grafts on hematopoietic reconstitution remains unclear.

Methods

The two-color ISHAGE protocol represents the current gold standard for CD34⁺ cell enumeration but includes only the number of viable CD45⁺/CD34⁺ cells relative to the body weight of the recipient. We adapted a multicolor flow cytometry marker panel for advanced character

SUBMITTER: Heuer A 

PROVIDER: S-EPMC10601604 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature