Spatial transcriptomics map of the embryonic mouse brain - a tool to explore neurogenesis.

ABSTRACT: The developing brain has a well-organized anatomical structure comprising different types of neural and non-neural cells. Stem cells, progenitors and newborn neurons tightly interact with their neighbouring cells and tissue microenvironment, and this intricate interplay ultimately shapes the output of neurogenesis. Given the relevance of spatial cues during brain development, we acknowledge the necessity for a spatial transcriptomics map accessible to the neurodevelopmental community. To fulfil this need, we generated spatially resolved RNA sequencing (RNAseq) data from embryonic day 13.5 mouse brain sections immunostained for mitotic active neural and vascular cells. Unsupervised clustering defined specific cell type populations of diverse lineages and differentiation states. Differential

SUBMITTER: Di Marco B 

PROVIDER: S-EPMC10602001 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature