Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.

ABSTRACT: The ability to image at high speeds is necessary in biological imaging to capture fast-moving or transient events or to efficiently image large samples. However, due to the lack of rigidity of biological specimens, carrying out fast, high-resolution volumetric imaging without moving and agitating the sample has been a challenging problem. Pupil-matched remote focusing has been promising for high NA imaging systems with their low aberrations and wavelength independence, making it suitable for multicolor imaging. However, owing to the incoherent and unpolarized nature of the fluorescence signal, manipulating this emission light through remote focusing is challenging. Therefore, remote focusing has been primarily limited to the illumination arm, using polarized laser light for facilitating co

SUBMITTER: Dibaji H 

PROVIDER: S-EPMC10602066 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature