Transcriptomic Analysis Pipeline (TAP) for quality control and functional assessment of transcriptomes.

ABSTRACT:

Background

RNA-sequencing (RNA-seq) has revolutionized the exploration of biological mechanisms, shedding light on the roles of non-coding RNAs, including long non-coding RNAs (lncRNAs), across various biological processes, including stress responses. Despite these advancements, there remains a gap in our understanding of the implications of different RNA-seq library protocols on comprehensive lncRNA expression analysis, particularly in non-mammalian organisms.

Results

In this study, we sought to bridge this knowledge gap by investigating lncRNA expression patterns in Drosophila melanogaster under thermal stress conditions. To achieve this, we conducted a comparative analysis of two RNA-seq library protocols: polyA + RNA capture and rRNA-depletion. Our approach involv

SUBMITTER: Boyd J 

PROVIDER: S-EPMC10602190 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature