Recovery of Vibrio cholerae polarized cellular organization after exit from a non-proliferating spheroplast state.

ABSTRACT: Vibrio cholerae, the causative agent of cholera epidemics, is a rod-shaped bacterium with a highly polarized cellular organization. It can survive harmful growth conditions by entering a non-proliferating spheroplast state, which involves loss of the cell envelope and polarity. How polarized rod organization cells are formed when the spheroplasts exit the non-proliferating state remains largely uncharacterized. To address this question, we investigated how L-arabinose-induced V. cholerae spheroplasts return to growth. We found that de novo morphogenesis started with the elimination of an excess of periplasm, which was immediately followed by cell elongation and the formation of cell branches with a diameter similar to that of normal V. cholerae cells. Periplasm elimination was driven by bi

SUBMITTER: Goudin A 

PROVIDER: S-EPMC10602287 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature