MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues.

ABSTRACT: Super-resolution optical imaging tools are crucial in microbiology to understand the complex structures and behavior of microorganisms such as bacteria, fungi, and viruses. However, the capabilities of these tools, particularly when it comes to imaging pathogens and infected tissues, remain limited. MicroMagnify (µMagnify) is developed, a nanoscale multiplexed imaging method for pathogens and infected tissues that are derived from an expansion microscopy technique with a universal biomolecular anchor. The combination of heat denaturation and enzyme cocktails essential is found for robust cell wall digestion and expansion of microbial cells and infected tissues without distortion. µMagnify efficiently retains biomolecules suitable for high-plex fluorescence imaging with nanoscale precision.

SUBMITTER: Cheng Z 

PROVIDER: S-EPMC10602566 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature