Promoter cloning and activities analysis of JmLFY, a key gene for flowering in Juglans mandshurica.

ABSTRACT: Juglans mandshurica (Manchurian walnut) is a precious timber and woody grain and oil species in Northeast China. The heterodichogamous characteristic phenomenon resulted in the non-synchronous flowering and development of male and female flowers, which limited the mating and the yield and quality of fruits. LFY is a core gene in the flowering regulatory networks, which has been cloned in J. mandshurica, and the function has also been verified preliminarily. In this study, the JmLFY promoter sequence with different lengths of 5'-deletion (pLFY1-pLFY6) were cloned and conducted bioinformatics analysis, the promoter activities were analyzed by detecting their driving activity to GUS gene in the tobacco plants that transformed with different promoter sequence stably

SUBMITTER: Zhang L 

PROVIDER: S-EPMC10602732 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature