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Trimodal single-cell profiling reveals a novel pediatric CD8αα⁺ T cell subset and broad age-related molecular reprogramming across the T cell compartment.

ABSTRACT: Age-associated changes in the T cell compartment are well described. However, limitations of current single-modal or bimodal single-cell assays, including flow cytometry, RNA-seq (RNA sequencing) and CITE-seq (cellular indexing of transcriptomes and epitopes by sequencing), have restricted our ability to deconvolve more complex cellular and molecular changes. Here, we profile >300,000 single T cells from healthy children (aged 11-13 years) and older adults (aged 55-65 years) by using the trimodal assay TEA-seq (single-cell analysis of mRNA transcripts, surface protein epitopes and chromatin accessibility), which revealed that molecular programming of T cell subsets shifts toward a more activated basal state with age. Naive CD4⁺ T cells, considered relatively resistant to aging,

SUBMITTER: Thomson Z

PROVIDER: S-EPMC10602854 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Dataset Information

Trimodal single-cell profiling reveals a novel pediatric CD8αα⁺ T cell subset and broad age-related molecular reprogramming across the T cell compartment.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Trimodal single-cell profiling reveals a novel pediatric CD8αα+ T cell subset and broad age-related molecular reprogramming across the T cell compartment.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Trimodal single-cell profiling reveals a novel pediatric CD8αα⁺ T cell subset and broad age-related molecular reprogramming across the T cell compartment.