Comparing extraction method efficiency for high-throughput palaeoproteomic bone species identification.

ABSTRACT: High-throughput proteomic analysis of archaeological skeletal remains provides information about past fauna community compositions and species dispersals in time and space. Archaeological skeletal remains are a finite resource, however, and therefore it becomes relevant to optimize methods of skeletal proteome extraction. Ancient proteins in bone specimens can be highly degraded and consequently, extraction methods for well-preserved or modern bone might be unsuitable for the processing of highly degraded skeletal proteomes. In this study, we compared six proteomic extraction methods on Late Pleistocene remains with variable levels of proteome preservation. We tested the accuracy of species identification, protein sequence coverage, deamidation, and the number of post-translational modific

SUBMITTER: Mylopotamitaki D 

PROVIDER: S-EPMC10603084 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature