Xrcc5/KU80 is not required for the survival or activation of prophase-arrested oocytes in primordial follicles.

ABSTRACT:

Introduction

The non-growing, meiotically-arrested oocytes housed within primordial follicles are exquisitely sensitive to genotoxic insults from endogenous and exogenous sources. Even a single DNA double-strand break (DSB) can trigger oocyte apoptosis, which can lead to accelerated depletion of the ovarian reserve, early loss of fertility and menopause. Therefore, repair of DNA damage is important for preserving the quality of oocytes to sustain fertility across the reproductive lifespan. This study aimed to evaluate the role of KU80 (encoded by the XRCC5 gene) - an essential component of the non-homologous end joining (NHEJ) pathway - in the repair of oocyte DNA DSBs during reproductive ageing, and following insult caused by the DNA-damaging chemotherapies cyclophosphamide and ci

SUBMITTER: Ratnayaka-Gamage ND 

PROVIDER: S-EPMC10603181 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature