Cleavable Cross-Linkers Redefined by a Novel MS³-Trigger Algorithm.

ABSTRACT: Cross-linking mass spectrometry (MS) is currently transitioning from a routine tool in structural biology to enabling structural systems biology. MS-cleavable cross-linkers could substantially reduce the associated search space expansion by allowing a MS³-based approach for identifying cross-linked peptides. However, MS² (MS/MS)-based approaches currently outperform approaches utilizing MS³. We show here that the sensitivity and specificity of triggering MS³ have been hampered algorithmically. Our four-step MS³-trigger algorithm greatly outperformed currently employed methods and comes close to reaching the theoretical limit.

SUBMITTER: Kolbowski L 

PROVIDER: S-EPMC10603603 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature