SpheroScan: a user-friendly deep learning tool for spheroid image analysis.

ABSTRACT:

Background

In recent years, 3-dimensional (3D) spheroid models have become increasingly popular in scientific research as they provide a more physiologically relevant microenvironment that mimics in vivo conditions. The use of 3D spheroid assays has proven to be advantageous as it offers a better understanding of the cellular behavior, drug efficacy, and toxicity as compared to traditional 2-dimensional cell culture methods. However, the use of 3D spheroid assays is impeded by the absence of automated and user-friendly tools for spheroid image analysis, which adversely affects the reproducibility and throughput of these assays.

Results

To address these issues, we have developed a fully automated, web-based tool called SpheroScan, which uses the deep learning framework called

SUBMITTER: Akshay A 

PROVIDER: S-EPMC10603766 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature