Extracellular Vesicle Refractive Index Derivation Utilizing Orthogonal Characterization.

ABSTRACT: The analysis of small particles, including extracellular vesicles and viruses, is contingent on their ability to scatter sufficient light to be detected. These detection methods include flow cytometry, nanoparticle tracking analysis, and single particle reflective image sensing. To standardize measurements and enable orthogonal comparisons between platforms, a quantifiable limit of detection is required. The main parameters that dictate the amount of light scattered by particles include size, morphology, and refractive index. To date, there has been a lack of accessible techniques for measuring the refractive index of nanoparticles at a single-particle level. Here, we demonstrate two methods of deriving a small particle refractive index using orthogonal measurements with commercially avail

SUBMITTER: Pleet ML 

PROVIDER: S-EPMC10603804 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature