Modernized Machine Learning Approach to Illuminate Enzyme Immobilization for Biocatalysis.

ABSTRACT: Biocatalysis is an established technology with significant application in the pharmaceutical industry. Immobilization of enzymes offers significant benefits for commercial and practical purposes to enhance the stability and recyclability of biocatalysts. Determination of the spatial and chemical distributions of immobilized enzymes on solid support materials is essential for an optimal catalytic performance. However, current analytical methodologies often fall short of rapidly identifying and characterizing immobilized enzyme systems. Herein, we present a new analytical methodology that combines non-negative matrix factorization (NMF)-an unsupervised machine learning tool-with Raman hyperspectral imaging to simultaneously resolve the spatial and spectral characteristics of all individual s

SUBMITTER: Wei H 

PROVIDER: S-EPMC10604017 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature